Hematologic Malignancies and Immune Monitoring Using Flow Cytometry

This project employs advanced flow cytometry technologies to investigate hematologic malignancies and assess immune responses in cancer patients. An advanced DEXI Flex flow cytometer (Beckman Coulter) equipped with 13 fluorescence filters is used for high-precision analysis of cellular characteristics and biomarkers. In addition, a BD Accuri flow cytometer with a 4- fluorescence filters is utilized for teaching and training purposes, supporting capacity building in flow cytometry applications.

Research Objectives:

• Hematologic Malignancies:
• Analysis of cellular and immunophenotypic profiles in:
• Acute Lymphoblastic Leukemia (ALL)
• Chronic Lymphocytic Leukemia (CLL)
• Acute Myeloid Leukemia (AML)
• Chronic Myeloid Leukemia (CML)
• Investigation of immune status by monitoring CD4⁺ and CD8⁺ T-cell populations Assessment performed
• pre- and post-chemotherapy to evaluate treatment-related immune alterations.