This project employs advanced flow cytometry technologies to investigate hematologic malignancies and assess immune responses in cancer patients. An advanced DEXI Flex flow cytometer (Beckman Coulter) equipped with 13 fluorescence filters is used for high-precision analysis of cellular characteristics and biomarkers. In addition, a BD Accuri flow cytometer with a 4- fluorescence filters is utilized for teaching and training purposes, supporting capacity building in flow cytometry applications.

Research Objectives:

• Hematologic Malignancies:
• Analysis of cellular and immunophenotypic profiles in:
• Acute Lymphoblastic Leukemia (ALL)
• Chronic Lymphocytic Leukemia (CLL)
• Acute Myeloid Leukemia (AML)
• Chronic Myeloid Leukemia (CML)
• Investigation of immune status by monitoring CD4⁺ and CD8⁺ T-cell populations Assessment performed
• pre- and post-chemotherapy to evaluate treatment-related immune alterations.

This project aims to prepare human amniotic membrane and utilize it as a biological dressing for the treatment of burns, chronic wounds, diabetic foot ulcers, and selected ocular disorders. The project focuses on evaluating the effects of different processing conditions on the amniotic membrane, including pre- and post-dehydration states and exposure to gamma irradiation at varying doses.

Special emphasis is placed on assessing structural integrity, cellular viability, and functional properties of the membrane following processing, in order to determine optimal conditions for safe clinical application.

The amniotic membrane is distinguished by its rich content of

• Stem cells and progenitor cells
• Epithelial and stromal tissue cells
• Bioactive and nourishing compounds with low immunogenicity

Project Objectives

• To prepare the amniotic membrane using sterile, safe, and standardized processing methods.
• To produce amniotic membrane based biological dressings that promote accelerated wound healing and tissue regeneration.